Fribrinogen Assay Coagulation
Quantitative measurement of fibrinogen in blood
Standard Parameters: BLANK TIME 1.0 sec.
(Criteria) ACTIVATION TIME 0.0 sec.
MAXIMUM TIME 60 sec.
Criteria LED Display: B= 1 A= 0 M= 60
Reagents: To perform the Fibrinogen assay you will use Thrombin reagent. Do not prewarm, keep at room temperature.
Fibrinogen Calibration Reference – Reconstitute with 1.0 ml of distilled water. Use in preparation of the calibration curve. Make dilutions of the reference in Veronal Buffer using the following dilution scheme:
|Reference Dilution #||Reference Plasma (ml)||Buffer
|2||1.0 of dil. #1||1.0||1:10||1.0|
|3||1.0 of dil. #2||1.0||1:20||0.5|
|4||1.0 of dil. #3||1.0||1:40||0.25|
- Assay your reference plasma dilutions first starting with the highest dilution (1:40). Testing of the dilutions for the reference curve must be accomplished within 30 minutes due to the instability of the samples.
- Calculate the “Fibrinogen mg/dl” to be used for your curve by using the dilution factor and the amount (mg/dl) of fibrinogen for your reference plasma. The concentration of fibrinogen (mg/dl) for the dilution is determined by multiplying the assigned value, designated on the vial label of the Fibrinogen Calibration Reference, by the dilution factor.
Example: Assume the assigned value for fibrinogen is 230 mg/dl; for a plasma dilution of 1:5 the concentration of fibrinogen is calculated as follows:
230 mg/dl x 2.0 = 460 mg/dl