Fribrinogen Assay Coagulation
FIBRINOGEN ASSAY
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Fribrinogen Assay Coagulation
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Quantitative measurement of fibrinogen in blood
Standard Parameters: BLANK TIME 1.0 sec.
(Criteria) ACTIVATION TIME 0.0 sec.
MAXIMUM TIME 60 sec.
Criteria LED Display: B= 1 A= 0 M= 60
Reagents: To perform the Fibrinogen assay you will use Thrombin reagent. Do not prewarm, keep at room temperature.
Fibrinogen Calibration Reference – Reconstitute with 1.0 ml of distilled water. Use in preparation of the calibration curve. Make dilutions of the reference in Veronal Buffer using the following dilution scheme:
DILUTION SCHEME
| Reference Dilution # | Reference Plasma (ml) | Buffer
(ml) |
Dilution | Dilution
Factor |
Fibrinogen
mg/dl |
| 1 | 0.5 | 2.0 | 1:5 | 2.0 | |
| 2 | 1.0 of dil. #1 | 1.0 | 1:10 | 1.0 | |
| 3 | 1.0 of dil. #2 | 1.0 | 1:20 | 0.5 | |
| 4 | 1.0 of dil. #3 | 1.0 | 1:40 | 0.25 |
- Assay your reference plasma dilutions first starting with the highest dilution (1:40). Testing of the dilutions for the reference curve must be accomplished within 30 minutes due to the instability of the samples.
- Calculate the “Fibrinogen mg/dl” to be used for your curve by using the dilution factor and the amount (mg/dl) of fibrinogen for your reference plasma. The concentration of fibrinogen (mg/dl) for the dilution is determined by multiplying the assigned value, designated on the vial label of the Fibrinogen Calibration Reference, by the dilution factor.
Example: Assume the assigned value for fibrinogen is 230 mg/dl; for a plasma dilution of 1:5 the concentration of fibrinogen is calculated as follows:
230 mg/dl x 2.0 = 460 mg/dl
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