Recombinant DNA Technology: Plasmid Vectors
Create a recombinant plasmid by using the plasmid DNA pages (white, pink and blue) from your lab kit. You will insert a “human gene” from the cellular DNA (pink paper) into a circular plasmid (white paper) using restriction enzymes (blue paper). Remember that your final product needs to have an origin of replication (to be able to replicate) and a selective marker (to find bacteria that are transformed) in addition to your gene of interest. Step-by-step instructions are attached.
Here is a short animation about how to create a plasmid vector: https://www.youtube.com/watch?v=KRpik9mNRm0
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Answer the questions in the attached document AND attach a photo of your plasmid, showing that it has the origin of replication, selective marker and human gene inserted.
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